Segmentation d'image par déformation multirésolution sur bases d'ondelettes combinant données et modèle

- Nous proposons une nouvelle méthode de segmentation par ajustement d'un modèle actif multirésolution à chaque niveau de résolution de données décomposées sur une base d'ondelettes orthogonales. La combinaison de l'approche multirésolution sur bases d'ondelettes, simultanément sur les données et sur le modèle, apporte une convergence rapide aux plus hautes résolutions. La solution obtenue est plus robuste et plus précise en présence de bruit que celle obtenue par un ajustement de contour actif non multirésolution

Visualization and Analysis of 3D Microscopic Images

In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain

Bringing privacy to the janus webrtc server: The perc way

This paper discusses how to add privacy features to the Janus WebRTC media server and gateway. In order to do so, we embrace a standard approach, by leveraging work currently ongoing within the IETF PERC working group. PERC stands for Privacy Enhanced RTP Conferencing and is aimed at enabling centralized real-Time conferencing in a scenario where the central media forwarding entity is not required to be trusted by any of the conference participants. The paper analyzes the impact of the integration of double encryption, which is part of the PERC efforts, both on the Janus core and on the plugins it provides for WebRTC streams management and manipulation

A T cell receptor associated with naturally occurring human tumor immunity

The onconeural antigens appear to serve as tumor rejection antigens in the paraneoplastic neurologic disorders. Here, we used an unbiased peptide binding screen, followed by studies in HLA-A2.1 transgenic mice to identify naturally processed HLA-A2.1 restricted epitopes of the paraneoplastic cerebellar degeneration breast/ovarian cancer antigen cdr2. These mice were used to clone high-avidity cdr2-specific CD8+ T cells that recognize human tumor cells presenting endogenously loaded MHC class I-cdr2 peptide. T cells with this specificity were detected in the peripheral blood of two HLA-A2.1+ paraneoplastic cerebellar degeneration patients. We cloned T cell receptor (TCR) α and β genes from cdr2-specific T cells; electroporation of RNA encoding this TCR turned nonreactive donor T cells into efficient killers of human cdr2-expressing tumor cells. Cloned cdr2-specific TCR genes provide a clinically relevant means for immunologic targeting of human gynecologic cancers